Jagged-1 Antibody [J1G53-3] | 36-051

Jagged-1 Antibody [J1G53-3] | 36-051 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human Jagged-1.

Research Area: Cancer

Tested Application: E, Flow, IHC, WB

Application: ELISA: (direct or indirect: 1:500-1:5, 000) . Flow Cytometry: (1:20-1:100) . Immunohistochemistry: (paraffin sections) . Western blot: (1:500-1:5, 000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

Specificiy: Recognizes human Jagged-1. Detects a band of ~180kDa by Western blot.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: J1G53-3

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: Protein Jagged-1; CD339; JAG1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro) . Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF) .