JAGN1 Antibody | 8367

JAGN1 Antibody | 8367 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (95%)

Immunogen: JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1.
The immunogen is located within the last 50 amino acids of JAGN1.

Research Area: Stem Cell, Immunology

Tested Application: E, WB, IHC-P

Application: JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist; this antibody will detect all but isoform 2.

Positive Control 1: Cat. No. 1411 - Mouse Colon Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 20 kDa
Observed: 25 kDa

Validation: N/A

Isoform: N/A

Purification: JAGN1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: JAGN1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: JAGN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Jagunal homolog 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1) . JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1) . Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2) .