JC-1 | 1130

JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.

JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.

1130 | JC-1 DataSheet

Alternate Name/Synonyms: 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide

Appearance: Brown red solid

Formulation: N/A

CAS Number: 3520-43-2

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₅H₂₇Cl₄IN₄

Molecular Weight: 652.23

Cell-Permeable?: Yes

Purity: >98% by HPLC

Solubilities: DMSO and DMF

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A mitochondrial dye

MDL Number: MFCD00188475

PubChem CID: 5492929

SMILES: CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]

InChi: InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30) 10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2, 1-4H3;1H/q+1;/p-1

InChi Key: FYNNIUVBDKICAX-UHFFFAOYSA-M