JMJD3 Antibody| F40550-0.05ML/F40552-0.08ML

JMJD3 Antibody| F40550-0.05ML/F40552-0.08ML | Gentaur UK, US & Europe Distribution

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Mouse

Application: WB, IHC, IF, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50

Application Note: Titration of the JMJD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This JMJD3 antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this JMJD3 antibody.

Storage: Aliquot the JMJD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A