JNK Activated Cell Lysate | 7032

Jurkat cells are cultured in RPMI medium supplement with 10% FBS. The cells were grown to late growing phase. Anisomycin is added to the medium to a final concentration 10 µg/ml ( cat# 1549-10, dissolved in DMSO to 10 mg/ml as stock). The cells are incubated at 37°C for 15 minutes, then collected and lysed in Extraction Buffer ( Cat # K430-40). The cell lysate can be used as a positive control for JNK activity assays in conjunction with ’s JNK Activity Assay Kit (K430-40 or K431-40). For positive controls, we recommend using 100 µg per assay.

7032 | JNK Activated Cell Lysate DataSheet

Sort Name: JNK Activated Cell Lysate

Label Name: N/A

Taglines: Anisomycin activated Jurkat cells extract for a positive control in western blot and JNK activity assays

Product Highlights: • CONCENTRATION- 4 mg/ml

Packaging: EA