Jurkat Cell Slide | 17-005

Jurkat Cell Slide | 17-005 | Gentaur UK, US & Europe Distribution

Type: Cell Line

Tested Application: ICC

Application: Jurkat cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.

Storage Condition: Jurkat cell slide can be stored for up to 6 months at 4˚C under desiccate conditions.

Background: Jurkat, Clone E6-1 (T lymphocyte; acute T cell leukemia)

Disclaimer: This product is intended for laboratory research purposes only and should be used by qualified personnel only.

Preparation: Human Jurkat, Clone E6-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The Jurkat cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Jurkat cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.

Additional Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs) .

Species: Human

General Diagnosis: Jurkat Cell Slide

Available Lots : human acute T cell leukemia

Fixation: 4% paraformaldehyde

Slide: 5 slides/pack

Lot: N/A