KIAA0319 Antibody | 46-866

KIAA0319 Antibody | 46-866 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat

Immunogen: The immunogen for this antibody is: C-QGKIKQENKPTLH

Research Area: Neuroscience

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Brain lysates (calculated MW of 118kDa according to Mouse NP_001074520.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17 (6) :859-71; PMID: 18063668) . Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Specificiy: The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1) , isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1) .

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: mKIAA0319, similar to KIAA0319 gene product, 4930451E12Rik, KIAA0319

User Note: N/A

BACKGROUND: N/A