KIRREL2 Antibody | 7971

KIRREL2 Antibody | 7971 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: KIRREL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KIRREL2.
The immunogen is located within amino acids 550 - 600 of KIRREL2.

Research Area: Neuroscience, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: KIRREL2 antibody is human, mouse and rat reactive. At least five isoforms are are known to exist. This antibody is predicted to not cross-react with other members of the KIRREL protein family.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 11-101 - Human Pancreas Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 64, 70, 78 kDa
Observed: 70 kDa

Validation: N/A

Isoform: N/A

Purification: KIRREL2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: KIRREL2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: KIRREL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Kin of irregular chiasm-like protein 2, Kin of IRRE-like protein 2, NEPH3, Nephrin-like protein 3, NLG1, FILTRIN

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: KIRREL2, also known as nephrin-like 3, is a type I transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules (1) . KIRREL2 consists of five extracellular Ig-like repeats, a transmembrane domain, several glycosylation sites and a cytoplasmic domain that has a stretch of nine conserved residues (1, 2) . KIRREL2 has been found to be expressed in beta cells of pancreatic islets and is involved in the regulation of both glomerular and neural development (3, 4) . Downregulation of KIRREL2 is implicated in several proteinuric diseases (4, 5) .