KIRREL3 Antibody | 7973

KIRREL3 Antibody | 7973 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3.
The immunogen is located within amino acids 20 - 70 of KIRREL3.

Research Area: Neuroscience, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 64, 84 kDa
Observed: 60 kDa

Validation: N/A

Isoform: N/A

Purification: KIRREL3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: KIRREL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Kin of irregular chiasm-like protein 3, Kin of IRRE-like protein 3, MRD4, KIRRE, NEPH2, PRO4502, Nephrin-like protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1) . KIRREL3 is involved in the regulation of both glomerular and neural development (2) , and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3) . KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4) .