KIT / CD117 Antibody | 43-381

KIT / CD117 Antibody | 43-381 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: KRDSFICSKQEDH

Research Area: Other

Tested Application: E, WB, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1) . An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 110kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian

User Note: N/A

BACKGROUND: N/A