KL-6 (Human) ELISA Kit - E4892

E4892 | KL-6 (Human) ELISA Kit DataSheet

Krebs von den Lungen-6 (KL-6) is a high molecular weight glycoprotein that belongs to the MUC-1 family. Moderate KL-6 expression is observed in type II pneumocytes and respiratory bronchiolar epithelial cells, whereas weak KL-6 expression is observed in basal cells of the terminal bronchiolar epithelium in normal lung tissues. Several studies reported increased KL-6 levels in regenerated type II alveolar epithelial cells and the serum of patients suffering from interstitial lung disease (ILD). Hence, KL-6 could serve as an efficient therapeutic target for the treatment of pulmonary diseases such as idiopathic pulmonary fibrosis, CTD-associated ILD, cystic fibrosis, and hypersensitivity pneumonitis. ’s KL-6 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of KL-6 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of KL-6 in the sample or standard.

Alternate Name : EMA, MCD, PEM, PUM, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, MUC1

Tag Line : A Sandwich ELISA kit to quantitatively measure KL-6 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma,Serum,Tissue lysate,Other biological fluids

Species Reactivity : Human

Applications : This Sandwich ELISA kit is designed to quantitatively measure amount of KL-6 in human serum, plasma, tissue lysates, and other biological fluids

Features and Benefits : This Sandwich ELISA is highly sensitive and highly specific for the detection of KL-6 in human samples. There is no significant cross-reactivity or interference between KL-6 and analogues,Detection range: 0.156 - 10 ng/ml,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Recovery range: 85 - 105% for normal human serum and plasma samples,Sensitivity: 0.094 ng/ml