KLH20 Antibody (C-term) [APR32163G]

KLH20 Antibody (C-term) [APR32163G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This KLH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 412-439 amino acids from the C-terminal region of human KLH20.

Cellular Localisation: Cytoplasm, perinuclear region. Nucleus. Golgi apparatus, trans-Golgi network. Cell projection, axon Cell projection, dendrite. Note=Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN- gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination (PubMed:20389280)

Molecular Weight: 67955

Clone: Polyclonal

Gene Name: KLHL20

Gene ID: 27252

Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR (KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280) . The BCR (KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed:24768539) . Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539) . Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR (KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed:21670212) . In case of tumor, the BCR (KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR (KLHL20) complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed:21840486) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Kelch-like protein 20, Kelch-like ECT2-interacting protein, Kelch-like protein X, KLHL20, KLEIP