KLOTHO Antibody | 6107

KLOTHO Antibody | 6107 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-KLOTHO antibody (6107) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO.
The immunogen is located within amino acids 410-460 of KLOTHO.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Three isoforms of KLOTHO are known to exist.

Positive Control 1: Cat. No. 1203 - A549 Cell Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 4: Cat. No. 1282 - 3T3/NIH Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 116kD
Observed: 116kD

Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar KLOTHO expression profile in human and mouse cell lines detected by two independent anti-KLOTHO antibodies that recognize different epitopes, 6107 against central domain and competitor antibody against human recombinant protein.  KLOTHO proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 2) : Anti-KLOTHO antibodies (6107) detected human KLOTHO recombinant protein at different concentrations.

Isoform: Human KLOTHO has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD) . Mouse KLOTHO has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD) . Rat KLOTHO has one isoform (1014aa, 117kD) . 6107 can detect human, mouse and rat.

Purification: KLOTHO Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: KLOTHO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: KLOTHO Antibody: Klotho

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.