Klotho, Human Recombinant | P1280

Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively. The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished. Recombinant human Klotho is a 65-70 kDa glycoprotein containing 516 amino acid residues.

P1280 | Klotho Human Recombinant DataSheet

Biomolecule/Target: Klotho

Synonyms: Klotho (EC:3.2.1.31), KL, Klotho, KLA

Alternates names: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.

Taglines: Glycosylated protein playing important role in the regulation of phosphate and calcium homeostasis

NCBI Gene ID #: 3082

NCBI Gene Symbol: HGF

Gene Source: Human

Accession #: P14210

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 EU/g

Activity (Specifications/test method): N/A

Biological activity: ED is typically 10 to 50 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.

Results: 10 to 50 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 70 kDa, single chain, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 15 mM Sodium Phosphate pH 7.3.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A