L-Asparaginase | P1321

L-Asparaginase is an enzyme that depletes L-Asparagine "an important nutrient for cancer cells" resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli, which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease. The rationale behind asparaginase is that it takes advantage of the fact that all cells are unable to synthesize the non-essential amino acid asparagine whereas normal cells are able to make their own asparagine. These leukemic cells depend on circulating asparagine. Asparaginase however catalyzes the conversion of L-asparagine to aspartic acid and ammonia. This deprives the leukemic cell of circulating asparagine.

P1321 | L-Asparaginase DataSheet

Biomolecule/Target: L-Asparaginase

Synonyms: L-asparagine amidohydrolase, L-ASNase, Escherichia coli L-asparaginase, L-ASP, Colaspase, ASP-1, asparaginase II

Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a.

Taglines: It is an anti-tumor agent derived from E.coli, which can inhibit the growth of malignant cells

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2

Gene Source: Human

Accession #: P01563

Recombinant: No

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 96.0%

Assay #2: RP-HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.1 to 0.4 ng/mL. The specific activity was determined by the dose-dependent cytotoxicity of the human TF -1 cell line(human erythroleukemic indicator cell line).

Results: 0.1 to 0.4 ng/ml

Binding Capacity: N/A

Unit Definition: One unit of enzyme catalyzes hydrolyzation of 10 nanomoles of dUTP to dUMP in one hour at 85°C.

Molecular Weight: 16 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder

Physical form description: The enzyme was lyophilized with no additives

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A