L-Methionine -Lyase, Pseudomonas putida recombinant | 7848

Methionine gamma-lyase (EC 4.4.1.11) from Pseudomonas putida is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism. METase catalyzes the , -elimination of methionine to -ketobutyrate, methanethiol, and ammonia. METase also catalyzes the , -elimination of other sulfur containing amino acids, such as homocysteine, cysteine. Because of its ability to deplete methionine, METase has been considered as a viable component of cancer therapeutics against methionine-dependent tumor cells. METase has also been utilized to design drug targets for the infectious diseases caused by parasitic protozoa and anaerobic periodontal bacteria.

7848 | L-Methionine -Lyase Pseudomonas putida recombinant DataSheet

Biomolecule/Target: L-Methionine -Lyase

Synonyms: L-Methionine--deamino--mercaptomethane-Lyase, Methioninase, METase, MGL, mdeA

Alternates names: IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.

Taglines: 90% Pure Active Recombinant L-Methionine -Lyase which plays a central role in sulfur amino acid metabolism

NCBI Gene ID #: 3569

NCBI Gene Symbol: IL6

Gene Source: Human

Accession #: P05231

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: > 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level:

Activity (Specifications/test method): TF-1 cells were stimulated with human recombinant IL-6 for 36 hrs and cell proliferation was measured using the Quick Cell Proliferation Colorimetric Assay Kit Plus (Cat. No. K302).

Biological activity: EC50 < 1 ng/mL

Results: EC50 <0.7 ng/mL

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme required to convert 1.0 mole of L-methionine to -ketobutyrate, methanethiol and ammonia per minute in 20 mM potassium phosphate, pH 8.3, 150 mM NaCl at 37 °C

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of 22.8 kDa (30-212aa). Under reducing conditions the protein migrates as ~23-29 kDa bands in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 10 mg/ml in 20 mM potassium phosphate, 150 mM NaCl, pH 8.3.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water.

Amino acid sequence: N/A