Lactoferrin (Human) ELISA Kit - E4726

E4726 | Lactoferrin (Human) ELISA Kit DataSheet

Lactoferrin is a glycoprotein present in exocrine secretions and in the secondary granules of polymorphonuclear neutrophils. It is the iron-binding protein in milk. Lactoferrin is known to regulate intestinal iron absorption, and to participate in the defense against bacteria. Lactoferrin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human Lactoferrin. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for human Lactoferrin and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human Lactoferrin. The concentration of Human Lactoferrin in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : LTF, GIG12, HLF2, LF, Lactotransferrin

Tag Line : A Sandwich ELISA kit for the quantitative measurement of Lactoferrin in human serum, plasma, culture media samples

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma, Serum, Other biological fluids

Species Reactivity : Human

Applications : Sensitivity: 0.19 ng/ml, in vitro quantitative determination of Human LTF/LF concentrations in serum, plasma and other biological fluids., Coefficient of variation is < 10%, Detection Range: 0.31-20 ng/ml, Specificity: This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed.

Features and Benefits : Coefficient of variation is < 10%., Detection Range: 0.31-20 ng/ml, Sensitivity: 0.19 ng/ml, Specificity: This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed.