LAMB1 Antibody | 23-927

LAMB1 Antibody | 23-927 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human LAMB1 (NP_002282.2) .

Research Area: Cell Cycle, Signal Transduction

Tested Application: WB, IHC, IF

Application: WB: 1:1000 - 1:4000
IHC: 1:10 - 1:200
IF: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: SW480

Positive Control 2: HepG2

Positive Control 3: HT-1080

Positive Control 4: SKOV3

Positive Control 5: Mouse heart

Positive Control 6: N/A

Molecular Weight: Observed: 198kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: LAMB1, CLM, Laminin B1 chain, Laminin, beta 1, Laminin-12 subunit beta, Laminin-6 subunit beta, Laminin-1 subunit beta, Laminin-8 subunit beta, Laminin beta-1, Laminin beta-1 chain, Laminin subunit beta-1, Laminin-10 subunit beta, Laminin-2 subunit beta

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.