LARP7 Antibody (C-term) [APR31054G]

LARP7 Antibody (C-term) [APR31054G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This LARP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the C-terminal region of human LARP7.

Cellular Localisation: Nucleus, nucleoplasm

Molecular Weight: 66899

Clone: Polyclonal

Gene Name: LARP7 {ECO:0000303|PubMed:18483487, ECO:0000312|HGNC:HGNC:24912}

Gene ID: 51574

Function: RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function (PubMed:18249148, PubMed:32017898) . Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II (PubMed:18249148, PubMed:18483487) . The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:18249148, PubMed:18483487) . The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838) . LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex (PubMed:18483487, PubMed:18281698) . LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing (PubMed:32017898) . Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes (PubMed:32017898) . U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898) . Binds U6 snRNAs with a 5'- CAGGG-3' sequence motif (PubMed:32017898) . U6 snRNA processing is required for spermatogenesis (By similarity) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: La-related protein 7, La ribonucleoprotein domain family member 7, P-TEFb-interaction protein for 7SK stability, PIP7S, LARP7