Additional Information
Size:  | 
                                                    100 µg  | 
                                                
Target:  | 
                                                    LC3A  | 
                                                
Conjugate:  | 
                                                    ATTO 700  | 
                                                
Research Area:  | 
                                                    Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart  | 
                                                
Alternative Name:  | 
                                                    MAP1 light chain 3 like protein 1 Antibody, Map1lc3a Antibody, MAP1BLC3 Antibody, Autophagy-related ubiquitin-like modifier LC3 A Antibody, MAP1LC3A Antibody, LC3A Antibody, Microtubule-associated proteins 1A and 1B, light chain 3 Antibody, MAP1ALC3  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal  | 
                                                
Immunogen:  | 
                                                    Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, ICC/IF  | 
                                                
Isotype:  | 
                                                    NA  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS, 50% glycerol, 0.09% sodium azide  | 
                                                
Concentration:  | 
                                                    1 mg/ml  | 
                                                
Specificity:  | 
                                                    Detects ~14 kDa. Band at 50 kDa is LC3A complex.  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:1000 dilution of SPC-613 was sufficient for detection of LC3A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-Anchor, Cytoplasmic Vesicle, Autophagosome Membrane, Lipid-Anchor, Cytoplasmic Vesicle, Autophagosome  | 
                                                
Accession Number:  | 
                                                    NP_115903.1  | 
                                                
Gene ID:  | 
                                                    84557  | 
                                                
Swiss-Prot:  | 
                                                    Q9H492  | 
                                                
Tissue Specificity:  | 
                                                    Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.  |