LEC Antibody [1.2_5G3-1D7] | XP-5722-M

LEC Antibody [1.2_5G3-1D7] | XP-5722-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hLEC as the immunizing antigen.

Research Area: Chemokines & Cytokines, Signal Transduction

Tested Application: E, WB

Application: ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.

Clonality: Monoclonal

Clone: 1.2_5G3-1D7

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: LEC lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: CCL16, CKb12, HCC4, ILINCK, LCC1, Liver expressed chemokine, LMC, Monotactin 1, Mtn1, NCC4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, C-C motif chemokine 16, Chemokine CC-4, HCC-4, LEC, LCC-1, Mtn-1, NCC-4

User Note: Centrifuge vial prior to opening.

BACKGROUND: LEC displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.