Leptin Antibody | XP-5213

Leptin Antibody | XP-5213 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Rat

Homology: N/A

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin) .

Research Area: Signal Transduction, Growth Factors, Obesity, Chemokines & Cytokines, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
To detect rat Leptin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rat Leptin.
Sandwich:
To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat Leptin (XP-5213Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.
Western Blot:
To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-rat Leptin specific antibody was purified by affinity chromatography employing immobilized rat Leptin matrix.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: Leptin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: OB, obese, Ob, Leptin, Obesity factor,

User Note: Centrifuge vial prior to opening.

BACKGROUND: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.