441

Leptospira 16s RNA RT-PCR (CE) | B49-FRT

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SKU:
441-B49-FRT-GEN
Availability:
IN STOCK
NULL740.00 - NULL950.00

Description

Leptospira 16s RNA RT-PCR (CE) | B49-FRT is available for delivery

Real Time Kit for the qualitative detection of Leptospira 16s RNA

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

Leptospira 16s RNA RT-PCR (CE) B49-FRT DataSheet

INTRODUCTION

Leptospirosis is a worldwide zoonosis caused by pathogenic species of the genus Leptospira. In 90% of cases, leptospirosis manifests as an acute febrile illness with a biphasic course and an excellent prognosis. Nonspecific signs and symptoms of leptospirosis (eg, fever, headache, nausea, vomiting) are often confused with viral illness. In 10% of cases, the presentation is more dramatic, and the infection has a mortality rate of 10%. Known as Weil disease or icteric leptospirosis, the classic definition of this forms of leptospirosis includes fever, jaundice, renal failure, and hemorrhage. Other organ systems (ie, pulmonary system, cardiac system, CNS) are also frequently involved.

INTENDED USE

The Leptospira 16s RNA Real-TM is a Real-Time test for the qualitative detection of Leptospira 16s RNA in the biological materials. Test contains an IC which serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition.

PRINCIPLE OF ASSAY

Leptospira 16s RNA Real-TM Test is based on three major processes: isolation of RNA from specimens, one-step reverse transcription of the RNA and Real Time amplification of the cDNA. Leptospira detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. Leptospira 16s RNA Real-TM PCR kit is a qualitative test which contains the Internal Control (IC). It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition

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Additional Information

Test Size:
50
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