Lhca4 | PSI type IV chlorophyll a/b-binding protein | AS01 008

Lhca4 | PSI type IV chlorophyll a/b-binding protein | AS01 008 | Gentaur UK, US & Europe Distribution

Immunogen: BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 2000-1 : 5000 (WB)

Molecular weight: 27.7 | 21 kDa for Arabidopsis thaliana

Confirmed Reactivity: Arabidopsis thaliana, Bryopsis corticulans, Citrus reticulata, Echinochloa crus-galli, Fortunella margarita Swingle, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pisum sativum, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays

Predicted Reactivity: Dicots, Gymnosperms

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein is processed into mature form (Jansson 1999) .

Background: The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G47470

Category: LHC

Research Area: Plant Biology, Photosynthesis