Lhcb2 | LHCII type II chlorophyll a/b-binding protein | AS01 003

Lhcb2 | LHCII type II chlorophyll a/b-binding protein | AS01 003 | Gentaur UK, US & Europe Distribution

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: AT2G05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:AT2G05070, Lhcb2.3 UniProt:Q9XF87, TAIR:AT3G27690

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), ImmunoGold (IG), Western blot (WB)

Related Products: AS01 002 | Anti-Lhcb3 | LHCII type III chlorophyll a/b-binding protein, rabbit antibodiesAS01 004 | Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, rabbit antibodiesAS13 2705 | Anti-Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated, rabbit antibodiesPlant protein extraction buffer

Recommended Dilutions: 5 µl of antibody solution (IP), 1: 100 (IG), 1: 500 - 1 : 5000 (WB)

Molecular weight: 28.6 | 25 kDa for Arabidopsis thaliana

Confirmed Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Arachis hypogaea, Brachypodium sylvaticum, Cicer arietum, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardtii, Cucumis sativus, Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L., Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lycopersicon esculentum (Solanum lycopersicon), Miscanthus x giganteus, Mesembryanthemum crystallinum, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus coccineus L., Phaseolus vulgaris, Physcomitrium patens, Sinapsis alba, Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Triticum aestivum, Triticale, Zea mays

Predicted Reactivity: Algae, Dicots, Gymnosperms, Mosses

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.Protein is processed into mature form (Jansson 1999) .

Background: The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29) : 9467-9476.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G05100

Category: LHC

Research Area: Plant Biology, Photosynthesis