Lhcb3 | LHCII type III chlorophyll a/b-binding protein | AS01 002

Lhcb3 | LHCII type III chlorophyll a/b-binding protein | AS01 002 | Gentaur UK, US & Europe Distribution

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb3 UniProt: Q9S7M0, TAIR:AT5G54270. This sequence is highly conserved even in Ginko biloba and one of the major LHCII-forms of Physcomitrella patens.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS01 002PRE | Lhcb3 | LHCII type III chlorophyll a/b-binding protein, pre-immune serumAS01 003 | Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, rabbit antibodiesAS01 004 | Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, rabbit antibodiesPlant protein extraction buffer

Recommended Dilutions: 1 : 2000 (WB)

Molecular weight: 28.7 | 26 kDa for Arabidopsis thaliana

Confirmed Reactivity: Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Cucumis sativa, Dactylis glomeRata, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomirella patens, Prasinoderma sp., Pyramimonas sp., Spinacia oleracea, Triticum aestivum, Triticale, Zea may, Verbascum lychnitis

Predicted Reactivity: Cucumis melo, Dicots, Gymnosperms, MossesSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein is processed into mature form (Jansson 1999) .

Background: The major light-harvesting antenna complex II (LHCII) in photsynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar and regularily present in multiple gene-copies, the Lhcb3 protein differs in pigment-composition and molecular size and often is coded by only a single gene. Lhcb3 seems not to be present in the mobile LHCII trimers involved in state 1-state 2 transitions. A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29) : 9467-9476.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G54270

Category: LHC

Research Area: Plant Biology, Photosynthesis