LIF, murine recombinant | 4924

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.

4924 | LIF murine recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Leukemia Inhibitory Factor

Alternates names: T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Taglines: A cytokine that affects cell growth by inhibiting differentiation

NCBI Gene ID #: 3558

NCBI Gene Symbol: IL2

Gene Source: Human

Accession #: P60568

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: EDis typically 0.2 to 2 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Results: 0.2 to 2 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from sterile solution containing 20 mM phosphate buffer, pH 7.4 and 0.02% Tween-20

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A