LIGHT Detection Set (Risk Free) | RF16060

LIGHT Detection Set (Risk Free) | RF16060 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: LIGHT antibodies were raised against the extracellular domain of human LIGHT.

Tested Application: E, WB, ICC, IHC-P, Flow, IF

Application: N/A

Specificity: N/A

Purification: Antibodies are supplied as protein A purified IgG2b.

Clonality: Monoclonal

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide.

Concentration: Antibody 1 mg/mL

Storage Condition: Mouse Monoclonal: Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year.

As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Background: LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

User Note: Optimal dilutions for each application to be determined by the researcher.