Lin28-TAT, Human Recombinant | P1010

Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells.

P1010 | Human CellExp Frizzled-2 / FZD2 Protein Human recombinant DataSheet

Biomolecule/Target: Lin28-TAT

Synonyms: CSDD1, LIN28A, ZCCHC1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1

Alternates names: Heat Shock Protein 90, HSP90

Taglines: Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis.

NCBI Gene ID #: 3326

NCBI Gene Symbol: HSP90AB1

Gene Source: Human

Accession #: P08238

Recombinant: Yes

Source: Sf9 cells

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): N/A

Biological activity: Not available at this time

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 83.4 kDa

Concentration: N/A

Appearance: Dry powder

Physical form description: Lyophilized powder

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHHSSGLVPRGSHMPEEVHHGEEEVETFPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD