Description
Listeria monocytogenes p60 Antibody [p6007] | 36-021 | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Listeria monocytogenes
Homology: N/A
Immunogen: Recombinant Listeria monocytogenes p60.
Research Area: Innate Immunity
Tested Application: E, WB
Application: ELISA: (direct or indirect: 1:5, 000-1:10, 000) . Western blot: (1:2, 000-1:10, 000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.
Specificiy: Recognizes L. monocytogenes p60 protein. Does not cross-react with other Listeria species.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: p6007
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.