Listeria sp. p60 Antibody [p6017] | 36-022

Listeria sp. p60 Antibody [p6017] | 36-022 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Listeria monocytogenes, Listeria sp.

Homology: N/A

Immunogen: Recombinant Listeria spp. p60.

Research Area: Innate Immunity

Tested Application: E, WB

Application: ELISA: (direct or indirect: 1:5, 000-1:10, 000) . Western blot: (1:2, 000-1:10, 000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

Specificiy: Recognizes L. monocytogenes p60 protein. Weakly cross-reacts with other Listeria species (L. innocua, L. ivanovii) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: p6017

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.