Live and Dead Cell Double Staining Kit | KTA1001

Live and Dead Cell Double Staining Kit | KTA1001 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cellular states

Product Type: Apoptosis Detection Kit

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Kit Component: • JC-1 Stain
• CCCP (10 mM)
• Assay Buffer (5×)

Features & Benefits : • Measure mitochondrial membrane potential as an indicator of cell health.
• Use JC-1, a fluorescent, lipophilic, cationic dye.
• Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health.
• Optimized for flow cytometery or fluorescent microscopy.
• Contain CCC (Potent mitochondrial oxidative phosphorylation uncoupler) .

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Usage Note: • JC-1 Stain is difficult to prepare due to its low solubility. Make sure JC-1 Stain is completely thawed and warmed to room temperature before using.
• JC-1 Stain is light senstive. Incubations need to be done in the dark.

Storage Conditions: Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.

Shipping: Gel pack with blue ice.

Background: Mitochondria, the site of most energy production in eukaryotic cells, have a double membrane structure: an outer membrane and a folded inner membrane. Across the inner membrane of intact mitochondria there is a voltage gradient with the inside negative and the outside positive. Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis.

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Tag: JC-1