LNPEP Antibody | 7055

LNPEP Antibody | 7055 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (73%) , Rat: (73%)

Immunogen: LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.
The immunogen is located within amino acids 900 - 950 of LNPEP.

Research Area: Immunology

Tested Application: E, WB, IF

Application: LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.

Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 113 kDa

Validation: N/A

Isoform: N/A

Purification: LNPEP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: LNPEP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: LNPEP Antibody: CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP) , was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.