LRRC8A Antibody (C-term) [APR04436G]

LRRC8A Antibody (C-term) [APR04436G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This LRRC8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782-810 amino acids from the C-terminal region of human LRRC8A.

Cellular Localisation: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Note=Mainly localizes to the cell membrane, with some intracellular localization to lysosomes

Molecular Weight: 94199

Clone: Polyclonal

Gene Name: LRRC8A {ECO:0000303|PubMed:22532330, ECO:0000312|HGNC:HGNC:19027}

Gene ID: 56262

Function: Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24725410, PubMed:29769723, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731) . The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24725410, PubMed:30095067, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731) . Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28193731) . LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471) . In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells (PubMed:33171122) . In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP (PubMed:33171122) . Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E) ; channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731) . Can form functional channels by itself (in vitro) (PubMed:26824658) . Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746) . Also required for T-cell development (By similarity) . Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity) . Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion (PubMed:29371604) . Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis (PubMed:31270356, PubMed:33139539) .

Summary: Tissue Location: Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells. Expressed on T- cells as well as on B-lineage cells.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Volume-regulated anion channel subunit LRRC8A, Leucine-rich repeat-containing protein 8A, Swelling protein 1, LRRC8A, KIAA1437, LRRC8, SWELL1