LRWD1 Antibody (N-term) [APR30848G]

LRWD1 Antibody (N-term) [APR30848G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This LRWD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LRWD1.

Cellular Localisation: Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8BUI3}. Chromosome, centromere, kinetochore. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3

Molecular Weight: 70861

Clone: Polyclonal

Gene Name: LRWD1

Gene ID: 222229

Function: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post- replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.

Summary: Tissue Location: Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, CENP-33, Origin recognition complex-associated protein, ORC-associated protein, ORCA, LRWD1, CENP33, ORCA