LSD1, Human Recombinant | P1659

The mono, di- and trimethylation of particular lysine residues in histone tails (e.g. histone H3 lysine-4 (H3K4), H3K9, H3K27, H3K36, H4K20) are implicated in the transmission of heritable epigenetic information and the control of chromatin structure and DNA transcription. LSD1 catalyzes the oxidative demethylation of mono- and dimethylated H3K4, producing hydrogen peroxide and formaldehyde in the process. H3K4 methylation is considered a transcription activating chromatin mark. In vivo LSD1 is frequently found in association with the transcriptional corepressor protein CoREST and HDACs 1 or 2. Its expression is elevated in a number of cancers, which makes it a promising target for drug development. LSD1 is inhibited by a number of established monoamine oxidase inhibitor drugs.

P1659 | LSD1, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Human Recombinant LSD,LSD, Human Recombinant,Human Recombinant LSD1,Lysine-specific histone demethylase, 1KDM1, BHC110, AOF2, KIAA0601, KDM1A, lysine (K)-specific demethylase 1A

Alternates names: Human Recombinant LSD,LSD, Human Recombinant,Human Recombinant LSD1,Lysine-specific histone demethylase, 1KDM1, BHC110, AOF2, KIAA0601, KDM1A, lysine (K)-specific demethylase 1A

Taglines: Active human recombinant Lysine (K)-specific demethylase 1 protein

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #: BAA25527

Recombinant: TRUE

Source: Sf9 insect cells

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 10 mU/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of LSD1 is the amount of enzyme that demethylates the Histone H3 Dimethyl Lysine4 Peptide substrate while generating 1 umol H2O2 per minute at pH 8.5 and 37 °C.

Molecular Weight: 93.9 kDa (His tag at the N-terminus)

Concentration: Lot specific

Appearance: Liquid protein

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans