LYAR Antibody (N-term) [APR05888G]

LYAR Antibody (N-term) [APR05888G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This LYAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-66 amino acids from the N-terminal region of human LYAR.

Cellular Localisation: Nucleus. Nucleus, nucleolus. Cytoplasm. Cell projection, cilium, photoreceptor outer segment {ECO:0000250|UniProtKB:Q08288}. Note=Component of pre- ribosomal particles, including pre-40S, pre-60S and pre-90S (PubMed:24495227) . Associated with cytoplasmic ribosomes, but not polysomes, as a component of the 60S subunit (PubMed:24990247) . In the retina, predominantly expressed in photoreceptor outer segments (By similarity) . In the nucleolus, colocalizes with nucleolin/NCL, therefore may reside in the dense fibrillar component (DFC) (By similarity) . {ECO:0000250|UniProtKB:Q08288, ECO:0000269|PubMed:24495227, ECO:0000269|PubMed:24990247}

Molecular Weight: 43615

Clone: Polyclonal

Gene Name: LYAR

Gene ID: 55646

Function: Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227) . Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918) . In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580) . Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918) . Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed:31413131) . In addition, inhibits NF-kappa-B-mediated expression of proinflammatory cytokines (PubMed:31413131) . Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity) . Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity) .

Summary: Tissue Location: Predominantly expressed in testis.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Cell growth-regulating nucleolar protein, LYAR