M TLR4 Antibody (N-term) [APR10938G]

M TLR4 Antibody (N-term) [APR10938G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of mouse TLR4.

Cellular Localisation: Cell membrane; Single-pass type I membrane protein. Early endosome {ECO:0000250|UniProtKB:O00206}. Cell projection, ruffle. Note=Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation {ECO:0000250|UniProtKB:O00206}

Molecular Weight: 95519

Clone: Polyclonal

Gene Name: Tlr4

Gene ID: 21898

Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:9851930, PubMed:9989976, PubMed:20133493) . Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:24380872) . Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL (-) ) and mediates the cytokine release induced by LDL (-) (By similarity) . Activated by the signaling pathway regulator NMI which acts as damage- associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (By similarity) .

Summary: Tissue Location: Expressed in macrophages (at protein level) (PubMed:28098138) . Highly expressed in heart, spleen, lung and muscle Lower levels are found in liver and kidney (PubMed:23812099)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Toll-like receptor 4, CD284, Tlr4, Lps