MAEA Antibody (N-term) [APR17269G]

MAEA Antibody (N-term) [APR17269G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MAEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-42 amino acids from the N-terminal region of human MAEA.

Cellular Localisation: Cytoplasm {ECO:0000250|UniProtKB:Q4VC33}. Nucleus, nucleoplasm. Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton. Note=Detected in a nuclear, speckled- like pattern (PubMed:16510120) . Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis (PubMed:16510120) Present in cytoplasm, nuclear matrix and at the cell surface in macrophages; predominantly nuclear in immature macrophages and predominantly detected at the cell surface in mature macrophages Colocalizes with F-actin in macrophages (By similarity) {ECO:0000250|UniProtKB:Q4VC33, ECO:0000269|PubMed:16510120}

Molecular Weight: 45287

Clone: Polyclonal

Gene Name: MAEA

Gene ID: 10296

Function: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972) . MAEA is required for normal cell proliferation (PubMed:29911972) . The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed:29911972) . Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (By similarity) . Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (PubMed:9763581) . Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity) . May contribute to nuclear architecture and cells division events (Probable) .

Summary: Tissue Location: Detected at macrophage membranes (at protein level) . Ubiquitous.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Macrophage erythroblast attacher, Cell proliferation-inducing gene 5 protein, Erythroblast macrophage protein, Human lung cancer oncogene 10 protein, HLC-10, MAEA, EMP