50

Magnesium assay kit (Xyridil Blue-I)

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SKU:
50-CMG-035-GEN
Availability:
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NULL706.00

Description

Magnesium assay kit (Xyridil Blue-I)

[ Product Description ]

Magnesium is one of the most abundant minerals in the living cells, and plays important roles in many metabolic reactions such as polyphosphoric acid formation and photosynthesis in the chlorophyll.

[ Assay principle ]

Under alkaline condition, Xyridyl Blue-I(XB-I) - magnesium complex shows absorbance at 520 nm, and on the other hand, the absorbance by free XB-I at 600-680nm decreases.

[ Performance ]

1. Sensitivity:
2. Accuracy:
3. Intra assay variation:
4. Assay range:		 O.D. at 2 mg/dL standard will be 0.05 - 0.1(660nm vs 700nm)
±15% of known concentration
C.V. is less than 5%
0.05 - 3.5 mg/dL

[ Specifications ]
Assay principle: XB-I method (Colorimetric assay at 520-660 vs 700 nm)
Measuring wavelength: 660nm (reference 700 nm) is recommended.
Measuring at 520 nm vs 700 nm shows higher sensitivity but may be susceptible to various factors.
Specifity: Magnesium (Mg)
Measuring range: 0.05 - 3.5 mg/dL
Assay conditions: 5 minutes at room temperature (25 - 37 °C)
Applications: Serum, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. This assay will be interfered by EDTA, hemoglobin, anticoagulants and high concentration of potassium.
Storage temperature: 2 - 8°C
Expiry date: 1 year after the day of manufacturing.
Required but not provided: Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader (measuring wavelength 660/700 nm)

 

[ Contents of this kit ]
R-1 Chelate color (ready to use): 25 mL
Magnesium standard 2 mg/dL (ready to use): 0.5 mL

 

[ References: Assay examples ]
1) Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M:
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
J Bacteriol 196(22),p3820-3830(2014) doi: 10.1128/JB.01896-14
2) Sakamoto A, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010
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Additional Information

Size:
100 Tests
Type of Marker:
Trace elements assay
Marker:
Mg
Storage:
2 - 8°C.
Colorimetric:
660 nm
Assay range:
0.05-3.5 mg/dL
Usage:
Urine, Serum, Tissue
Remark:
Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.
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