MANF Antibody | 4349

MANF Antibody | 4349 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%)

Immunogen: Anti-MANF antibody (4349) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human MANF.
The immunogen is located within the first 50 amino acids of MANF.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.125-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This antibody does not cross-react with CDNF.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 20kD
Observed: 26 kD

Validation:

Recombinant Protein Test (Figure 2) : Anti-MANF antibodies (4349) detected human MANF recombinant protein at different concentrations.

Induced Expression Validation (Figure 6) : MANF expression detected by anit-MANF antibodies was up-regulated by VPA treatment.

Isoform: Human MANF has one isoform (182aa, 21kD) . Mouse MANF has one isoform (179aa, 20kD) and Rat MANF also has one isoform (179aa, 20kD) . 4349 can detect human, mouse and rat.

Purification: MANF Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MANF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MANF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: MANF Antibody: ARP, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.