MAPKAPK2, Active | 7737

MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.

7737 | MAPKAPK2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: MAPKAPK, MAP kinase-activated protein kinase 2

Alternates names: MAPKAPK, MAP kinase-activated protein kinase 2

Taglines: Involved in many signaling cascades

NCBI Gene ID #: UniGene:Mm.766

NCBI Gene Symbol: CXCL9

Gene Source: N/A

Accession #: P18340

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.

Biological activity: The biological activity of murine MIG was determined by its ability to chemoattract human lymphocytes using a concentration range of 0.1-1.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 68.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can be diluted into other buffered solutions or store at –20°C for future use.

Amino acid sequence: N/A