MARK4 Antibody (C-term) [APR17322G]

MARK4 Antibody (C-term) [APR17322G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MARK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 611-641 amino acids from the C-terminal region of human MARK4.

Cellular Localisation: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Cell projection, dendrite. Note=Localized at the tips of neurite-like processes in differentiated neuroblast cells Detected in the cytoplasm and neuropil of the hippocampus

Molecular Weight: 82520

Clone: Polyclonal

Gene Name: MARK4

Gene ID: 57787

Function: Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942) . Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762) . Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945) . Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532) . Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999) . Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999) . Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532) . Reduces neuronal cell survival (PubMed:15009667) . Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity) . Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity) . Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942) .

Summary: Tissue Location: Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310) . Expressed at highest levels in brain and testis Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1, MARK4, KIAA1860, MARKL1