MBD1 Polyclonal Antibody | BS65932

MBD1 Polyclonal Antibody | BS65932 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat,

Application: IHC-P,

Application Range: IHC-P=1:100-500

Background: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) . The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ADAM32 Polyclonal Antibody detects endogenous levels of ADAM37 protein.

Molecular Weight: 60kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: CXXC 3; CXXC3; MBD 1; MBD1; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; PCM 1; PCM1; Protein containing methyl CpG binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2 (FGF 2) transcription; MBD1_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human MBD1:101-200/605

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: affinity purified by Protein A

Pathway: