MCF 7 Membrane Lysate | XBL-10442

MCF 7 Membrane Lysate | XBL-10442 | Gentaur UK, US & Europe Distribution

Tested Application: WB

Application: MCF 7 (Breast Adenocarcinima) cell membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

Storage Condition: Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

User Note:

Background:

Shipping: Dry Ice

Type: Cell Line

Physical State: Liquid

Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.

Concentration: 2 mg/mL or better

Background: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9) , MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Disclaimer: This product is for research use only.

Short Description: membrane breast adenocarcinoma

Source: MCF 7 (Human Breast Adenocarcinima) cell line

Tissus By Diagnosis: Diseased

Tissus By Developemental Stage : N/A

Tissus By Species: Human

Tissus/ Cell: MCF 7 (Human Breast Adenocarcinima)

Diagnostic: Tumor

Cancer Type: N/A

Cancer Type Ezpanded: N/A

Age: N/A

Sex: N/A

Race: N/A

Species: Human