Description
MCF7 Cell Slide | 17-012 | Gentaur UK, US & Europe Distribution
Type: Cell Line
Tested Application: ICC
Application: MCF7 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.
Storage Condition: MCF7 cell slide can be stored for up to 6 months at 4˚C under desiccate conditions.
Background: MCF7 (breast; epithelial; pleural effusion adenocarcinoma)
Disclaimer: This product is intended for laboratory research purposes only and should be used by qualified personnel only.
Preparation: Human MCF7 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The MCF7 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 MCF7 cells. A follow-up heat dehydration process was done to ensure the attachment of MCF7 cells and the stability of cellular proteins.
Additional Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs) .
Species: Human
General Diagnosis: MCF7 Cell Slide
Available Lots : human breast epithelial adenocarcinoma
Fixation: 4% paraformaldehyde
Slide: 5 slides/pack
Lot: N/A