mCherry Fluorescent Protein | 4993

mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. The protein is a 28.8 kDa monomer with 256 amino acids, pI: 6.23. Ex.= 587 nm (540-590 nm); Em.= 610 nm (550-650 nm). The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody ( Cat # 3998-100) or protein purification/removal by using Ni++ beads.

4993 | mCherry Fluorescent Protein DataSheet

Biomolecule/Target: N/A

Synonyms: mCherry

Alternates names: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.

Taglines: Fluorescent protein ideal for subcellular labeling/visualization

NCBI Gene ID #: 3605

NCBI Gene Symbol: IL17A

Gene Source: Human

Accession #: Q16552

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF Adult fibroblasts.

Results: 5 to 25 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 30 to 35 kDa, homodimer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Freeze Dried

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A