mCherry (rabbit antibody) | AS18 4179

mCherry (rabbit antibody) | AS18 4179 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant full length mCherry expressed and purified from E. coli.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.

Format: Lyophilized

Tested Application: Immunocytochemistry (ICC), Western blot (WB)

Related Products: AS16 3967 | Anti-mCherry, monoclonal antibodiesAS18 4178 | Anti-mCherry, chicken antibodiesAS15 2987 | Anti-GFP (affinity purified), rabbit antibodiesAS15 2999 | Anti-GFP (total IgG), rabbit antibodiesAS15 3000 | Anti-GFP (protein A purified) rabbit antibodiesAS15 2998 | Anti-GFP (total IgY), chicken antibodiesAS15 3002 | GFP (purified recombinant protein) other tag antibodies

Recommended Dilutions: 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

Molecular weight: N/A

Confirmed Reactivity: mCherry

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.

Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer) . The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals) .

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: GFP/YFP/RFP/CFP

Research Area: Tag Antibodies