Mcl-1 Antibody | 3477

Mcl-1 Antibody | 3477 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Mcl-1.
The immunogen is located within amino acids 220 - 270 of Mcl-1.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, ICC, IF

Application: Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1ES by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This Mcl-1 antibody detects isoforms Mcl-1L and Mcl-1ES.

Positive Control 1: Cat. No. 1207 - Raji Cell Lysate

Positive Control 2: Cat. No. 17-007 - Raji Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22, 39 kDa
Observed: 39 kDa

Validation: N/A

Isoform: N/A

Purification: Mcl-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Mcl-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Mcl-1 Antibody: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.