MCP-1 Antibody | 5225

Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.

5225 | MCP-1 Antibody DataSheet

Antibody Target: MCP-1/MCAF

Target Alternative Name: MCP1, MCP-1, MCP 1, monocyte chemoattracctant protein-1, monocyte chemoattracctant protein 1, monocyte chemoattracctant protein

Tag Line:

Category: MCP

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Mouse, rat

Immunogen Sequence: E. coli-expressed rat MCP-1 (a.a. 1-73)

Accession #: P14844

Gene ID: 24770

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-rat MCP-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Purification: Affinity purified

Application: Western blot, Neutralization

Positive Control:

Application And Usages: Western blot analysis (0.5-2 µg/ml). From researcher’s feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified